goat anti mouse kit (R&D Systems)

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R&D Systems goat anti mouse kit
Goat Anti Mouse Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 221 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+c+kit/Human%2FMouse+CD117%2Fc-kit+Antibody/pmc12821522-55-37-40
Average 94 stars, based on 221 article reviews

goat anti mouse kit - by Bioz Stars, 2026-09

94/100 stars

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Immunostaining:

Article Title: c-Kit signaling confers damage-resistance to sweet taste cells upon nerve injury
Article Snippet: .. The primary antibodies used for tissue and organoid immunostaining included the following: rabbit anti-Krt14 (1:500, ab181595, Abcam), rat anti-Krt8 (1:1 000, Troma-1, DSHB, Iowa City, IA, US), rabbit anti-Krt8 (1:1 000, ab53280, Abcam), guinea pig anti-Krt13 (1:500, BP5076, OriGene, Rockville, MD, US), rabbit anti-NTPdase2 (1:1 000, mN2-36L I6, CHUQ, Québec, Canada), guinea pig anti-Trpm5 (1:500, generated in our previous paper ), goat anti-Car4 (1:500, AF2414, R&D Systems, Minneapolis, MN, US), goat anti-c-Kit (1:400, AF1356, R&D Systems), rabbit anti-GNAT3 (1:1 000, sc-395, Santa Cruz, Dallas, TX, US), goat anti-GNAT3 (1:1 000, OAEB00418, Aviva system, San Diego, CA, US), and rabbit anti-PLCβ2 (1:500, generated in this study), rabbit anti-PGP9.5 (1:400, GTX109637, Gene Tex, Irvine, CA, US), and goat anti-AIF-1/Iba1 (1:400, NB100-1028, Novus biological, Centennial, CO, US). ..

Generated:

Labeling:

Article Title: Investigation of Novel c-Kit-expressing Smooth Muscle Cells in Murine Cecum
Article Snippet: .. Because the immunoreactivity for c-Kit has not been reported in this area, except in our previous report [ ], we carefully examined c-Kit immunoreactivity labeled by two antibodies, namely goat anti-c-Kit (AF1356, R&D) and rat anti-c-Kit (CL8936AP, Cedarlane) combined with c-Kit-copGFP fluorescence in the cecal musculature ( A, B). ..

Fluorescence:

Blocking Assay:

Article Title:
Article Snippet: Individual lobes were thawed, embedded in low melting agarose and sectioned using a vibratome to generate 250μm thick slices, which were blocked overnight in 0.2% Triton/1%BSA/10% donkey serum/ PBS. .. Slices were immunostained with goat anti-c-Kit (R&D systems) and rat antiLyve-1 (Thermo Fischer Scientific) for 2 days in blocking solution, washed overnight in PBS and stained with DyLight488 donkey anti-goat IgG and DyLight549 or DyLight649 donkey anti-rat IgG (Thermo Fischer Scientific). .. Whole-mount-stained slices were then washed in PBS and incubated overnight in RapiClear 1.52 (Sunjin Lab).

Staining:

Incubation:

Article Title: Investigation of Novel c-Kit-expressing Smooth Muscle Cells in Murine Cecum
Article Snippet: .. After preincubation with normal donkey serum (5% in PBS) for 1 hr, sections were incubated with the following antibodies at room temperature: goat anti-c-Kit (AF1356, R&D, 1:1000), rat anti-c-Kit (ACK4, CL8936AP, Cedarlane, 1:500), mouse anti-α-smooth muscle actin-Cy3 (1A4, C6198, Sigma, 1:3000), rat anti-smooth muscle myosin heavy chain (KM3669, 80058, Yamasa, 1:1000), rabbit anti-PGP9.5 (RA95101, UltraClone, 1:5000), rabbit anti-TMEM16A (ab53212, Abcam, 1:500), and goat anti-LRIG1 (AF3688, R&D, 1:1000) [ , ]. .. After sections were incubated overnight with primary antibodies, they were washed with PBS before incubation in the secondary antibody (Alexa Fluor-coupled donkey anti-IgG, Molecular Probes, 1:500) for 1 hr at room temperature.

Article Title: Interstitial cells of Cajal in W sh /W sh c-kit mutant mice
Article Snippet: .. The specimens were pre-incubated with normal donkey serum (5% in PBS) for 1 h. Then, the specimens were incubated overnight with the following antibodies: rat anti-c-Kit (ACK4; 1:700; ACL8936AP; Cedarlane), goat anti-c-Kit (1:1,000; AF1356, R&D), rabbit anti-TMEM16A (1:400; ab53212; Abcam), rat anti-platelet derived growth factor receptor alpha (PDGFRα, APA5; 1:500; 14-1401; eBioscience), and mouse anti-alpha smooth muscle actin (SMA)-Cy3 (1:3,000; 1A4, C6198; Sigma-Aldrich). .. After incubation with the aforementioned primary antibodies, they were washed with PBS before being incubated with Alexa Fluor-coupled donkey anti-IgG (1:500; Molecular Probes, USA), a secondary antibody, for 1 h. Then, the specimens were washed with PBS and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Molecular Probes) and mounted in Anti-Fade Fluorescence Mounting Medium-Aqueous, Fluoroshield (ab104135, Abcam).

Article Title: Single-Cell Transcriptomics Reveals Zone-Specific Alterations of Liver Sinusoidal Endothelial Cells in Cirrhosis
Article Snippet: .. Primary antibodies were incubated overnight at 4°C (rabbit anti-α-SMA, 1:300, ab124964; rabbit anti-CD34, 1:100, ab81289; rabbit anti-Ednrb, 1:100, ab117529; rabbit anti-Lyve1, 1:300, ab14917, [Abcam, Cambridge, MA]; rat anti-CD31, 1:100, 550274 [BD Pharmingen, San Diego, CA]; goat anti-VE-cadherin, 1:200, sc-6458 [Santa Cruz Biotechnology, Dallas, TX]; rabbit anti-vWF, 1:100, A0082 [Agilent Dako, Santa Clara, CA]; rabbit anti-CD36, 1:100, 18836-1-AP [Proteintech, Rosemont, IL]; goat anti-Thrombomodulin, 1:20, AF3894, goat anti-c-kit, 1:20, AF1356 [R&D Systems, Minneapolis, MN]). .. Then, secondary antibodies were incubated for 30 minutes at room temperature (donkey anti-rabbit Alexa 647, 1:300; donkey anti-rat Alexa 647, 1:300; donkey anti-goat Alexa 488, 1:300; Invitrogen).

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